3D Heteronuclear NMR techniques for carbon-13 in natural abundance

H. Kessler, P. Schmieder, H. Oschkinat

J. Am. Chem. Soc. (1990) 112, 8599-8600

New 3-dimensional heteronuclear NMR techniques are proposed which allow recording the spectra in natural abundance. The high resolution and relatively short measuring time makes them suitable for routine spectroscopy. The principle of the technique is the reduction of spectral width by multiplicity selection (CH2, CH3) via heteronuclear multiple quantum coherence and a TOCSY transfer to all protons coupled to these groups. The method is demonstrated with a sulfur analog of cyclosporin A.