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To obtain detailed
information on the structure of the chromophore in phytochromes proteins, its
interactions with the protein matrix as well as the mechanisms that finally
leads to signal transduction a variety of spectroscopic techniques are usually
applied. The various spectroscopic techniques rely, however, on the calculation
of the spectra by theoretical methods. Those calculations work best if they
have been fine tuned using model compounds that are preferably related to the
molecule that needs to be investigated. For the calculation of tetrapyroll
moieties, model compounds have been either tetrapyrolls in the crystal or
embedded in protein matrices. Structures in solution have, however, scarcely
been determined. Solution NMR should be the method of choice to obtain a
detailed structural picture of tetrapyrolls in solution.
In the present project we aim to obtain information on the
strcutre and dynamics of several tetrapyroll compounds in various solvents
using not only NOE s and coupling constnats but also RDCs and cross correlated
relaxation. The same compounds will be investigated using Raman spectroscopy in
the same solvents. |
